Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Shan Chi Hsieh
Date created: 2012-09-17 11:00:00
Date modified: 2015-05-08 01:13:28
gamma-delta resolvase cassette
| Types | DnaRegion |
| Roles | engineered_region Device |
| Sequences | BBa_K817018_sequence (Version 1) |
Description
These cassette is encodes an autoregulated resolvase(serine type recombinase)from E.coli F plasmid transposon tn1000(tn3 family). Its promoter region consists of 3 sub-sites(res site) and can process recombination. This cassette can resolve multimer that formed during replicative transposition, so it can also resolve plasmid multimer providing plasmid stability by avoiding dimer catastrophe. For this reason, it is multimer resolution system(MRS) that provide the same function as E.coli chromosome XerCD/dif but acts independent of cell cycle and may have higher efficiency on plasmids compared with slow XerCD system.Notes
The native sequence have two PstI site, these sites have been cleared by mutagenesis.Source
E.coli F plasmid transposon tn1000(tn3 family)| Sequence Annotation | Location | Component / Role(s) |
| tnpA tnpR tnpX resI-L resI-R resII-L resII-R resIII-L resIII-R PstI PstI recombination site | 1,110 274,825 841,888 156,163 176,184 206,214 230,238 244,252 260,268 53,53 612,612 169,169 | CDS feature/cds CDS feature/cds CDS feature/cds feature/binding non_covalent_binding_site feature/binding non_covalent_binding_site feature/binding non_covalent_binding_site feature/binding non_covalent_binding_site feature/binding non_covalent_binding_site feature/binding non_covalent_binding_site sequence_alteration feature/mutation feature/mutation sequence_alteration feature/dna sequence_feature |