pSBBs1C

BBa_K823021 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K823021

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Jara Radeck

Date created: 2012-08-15 11:00:00

Date modified: 2015-05-08 01:13:30

pSB_Bs1C-lacZ (lacZ reporter vector for B. subtilis)

Details

• Types: DnaRegion
• Roles: plasmid_vector; Plasmid_Backbone
• Sequences: BBa_K823021_sequence (Version 1)

Description

This part is a backbone vector for Bacillus subtilis. It integrates at the amyE locus. It has a Ampicillin resistance for cloning in E.coli and Chloramphenicol resistance for B.subtilis. The multiple cloning site is followed by a lacZ gene for reporter assays.
This BioBrick is part of the LMU 2012 igem project Bacillus BioBrick Box
http://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks

Notes

This reporter vector can be used with the normal BioBrick assembly strategies.

Source

This vector is the Bacillus vector pAC6 brought into the iGEM BioBrick standard.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
lacZ	49,3112	CDS, feature/cds
amyE'	3165,3698	sequence_feature, feature/misc
bla	4023,4883	CDS, feature/cds
'amyE	7402,8229	feature/misc, sequence_feature
cat	8813,9463	CDS, feature/cds

Other Properties

• igem#experience: Works
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K823021/1

Member of these Collections

• //chassis/multihost
• //chassis/prokaryote/bsubtilis
• //chassis/prokaryote/ecoli
• //plasmid/chromosomalintegration
• //plasmidbackbone/libraryscreening/promoter
• iGEM Parts Registry
• igem_plasmid_vectors