pSBBs4S

BBa_K823024 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K823024

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Jara Radeck

Date created: 2012-08-20 11:00:00

Date modified: 2015-05-08 01:13:30

pSB_Bs4S-P_xyl: Integrative expression vector for Bacillus subtilis

Details

• Types: DnaRegion
• Roles: Plasmid_Backbone; plasmid_vector
• Sequences: BBa_K823024_sequence (Version 1)

Description

This part is an expression vector for Bacillus subtilis. It integrates at the thrC locus. It has a ampicillin resistance for cloning in E.coli and chloramphenicol resistance for selection in B.subtilis. The multiple cloning site downstream of a Pxyl promoter, that is inducible with xylose.
This BioBrick is part of the LMU 2012 igem project Bacillus BioBrick Box http://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks

Notes

We removed any forbidden restriction sites by site-directed mutagenesis PCR. The clonging site also includes a rfp for selection for cloning in E.coli.

Source

This part is an igem compatible version of the Bacillus expression vector pXT

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
spc	211,885	feature/cds, CDS
'thrC	1028,1456	feature/misc, sequence_feature
thrB	1453,1863	feature/misc, sequence_feature
bla	2716,3576	CDS, feature/cds
hom	3731,4033	sequence_feature, feature/misc
thrC'	4033,4572	sequence_feature, feature/misc
Pxly region	4539,4763	promoter, feature/promoter

Other Properties

• igem#experience: Fails
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K823024/1

Member of these Collections

• //chassis/multihost
• //chassis/prokaryote/bsubtilis
• //chassis/prokaryote/ecoli
• //plasmid/chromosomalintegration
• //plasmid/expression
• //plasmidbackbone/expression/inducible
• iGEM Parts Registry
• igem_plasmid_vectors