Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jara Radeck
Date created: 2012-08-20 11:00:00
Date modified: 2015-05-08 01:13:30
pSBBs3C-luxABCDE (lux reporter vector for B.subtilis)
| Types | DnaRegion |
| Roles | Plasmid_Backbone plasmid_vector |
| Sequences | BBa_K823025_sequence (Version 1) |
Description
This part is a reporter vector for Bacillus subtilis. It integrates at the sacA locus of B.subtilis genome. It has a ampicillin resistance for cloning in E.coli and Chloramphenicol resistance for selection in B. subtilis. The multiple cloning site has a rfp for selection and is followed by the luxABCDE gene cluster for luminescence reporter assays.This BioBrick is part of the LMU-Munich 2012 igem project Bacillus BioBrickBOX http://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks
Notes
We removed any forbidden restriction sites by site-directed mutagenesis PCR. The clonging site also includes a rfp for selection for cloning in E.coli.Source
This vector is an igem compatible version of the pAH328 Bacillus reporter vecotr.| Sequence Annotation | Location | Component / Role(s) |
| luxA luxB luxC luxD luxE sacA bla sacA cat | 73,1155 1172,2155 2179,3621 3635,4558 4583,5695 5676,6515 6849,7721 8712,9503 9661,10323 | feature/cds CDS CDS feature/cds CDS feature/cds CDS feature/cds CDS feature/cds sequence_feature feature/misc CDS feature/cds sequence_feature feature/misc CDS feature/cds |