pSBBs2E

BBa_K823027 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K823027

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Jara Radeck

Date created: 2012-08-20 11:00:00

Date modified: 2015-05-08 01:13:30

pSB_Bs2E: Empty backbone for integration into Bacillus subtilis lacA locus

Details

• Types: DnaRegion
• Roles: plasmid_vector; Plasmid_Backbone
• Sequences: BBa_K823027_sequence (Version 1)

Description

This part is an empty backbone vector for the usage in Bacillus subtilis. It integrates in the lacA locus and can be selected with erythromycin + lincomycin (MLS). It has a ampicillin resistance for cloning in E.coli.

This BioBrick is part of the LMU 2012 Bacillus BioBrick Box project: http://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks

Notes

We removed any forbidden restriction sites by site-directed mutagenesis PCR. The clonging site also includes a rfp for selection in E.coli.

Source

This part is an igem compatible version of the Bacillus subtilis vector pAX01

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
erm	500,1300	CDS, feature/cds
lacA'	1600,2100	sequence_feature, feature/misc
bla	4603,5400	CDS, feature/cds
'lacA	5600,6100	feature/misc, sequence_feature

Other Properties

• igem#experience: Works
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K823027/1

Member of these Collections

• //chassis/multihost
• //chassis/prokaryote/bsubtilis
• //chassis/prokaryote/ecoli
• //plasmid/chromosomalintegration
• //plasmidbackbone/operation
• iGEM Parts Registry
• igem_plasmid_vectors