BBa_K824008

BBa_K824008 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K824008

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Steven Allen

Date created: 2012-09-30 11:00:00

Date modified: 2015-07-24 01:19:31

Cadmium Promoter with GFP Reporter

Details

• Types: DnaRegion
• Roles: engineered_region; Composite
• Sequences: BBa_K824008_sequence (Version 1)

Description

We digested the promoter responsive to Cadmium (BBa_K174015) with EcoRI and SpeI and digested the GFP plasmid (BBa_I13401) with XbaI and PstI. The promoter was Chloramphenicol resistant and the GFP was Ampicillin resistant. These digested fragments were mixed and ligated to the provided, linearized pSB3C1 plasmid. The ligation mix was grown under Chloramphenicol selection. The resulting colonies were tested for responsive GFP production from the addition of Cadmium and Arsenic, respectively.

Notes

none

Source

This part is a combination of 2007 Brown's iGEM part BBa_K174015 and MIT's part (from 2005) I13401.

Components

Access	Instance	Definition
public	BBa_K174015	BBa_K174015
public	BBa_I13401	BBa_I13401

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K174015	1,205	BBa_K174015
BBa_I13401	212,1068	BBa_I13401

Other Properties

• igem#experience: Works
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K824008/1

Member of these Collections

• //function/sensor/metal
• iGEM Parts Registry