DGAT

BBa_K836002 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K836002
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Ricardo Alvarado
Date created: 2012-09-17 11:00:00
Date modified: 2015-05-08 01:13:32

O-acyltransferase WSD from Acinetobacter sp. (codon usage optimized for R. opacus) as used



Types
DnaRegion

Roles
Coding

CDS

Sequences BBa_K836002_sequence (Version 1)

Description

This biobrick codifies for the protein DGAT (Diacylglycerol O-acyltransferase) which have dual activity, it can act as a wax ester synthase or as a diacylglycerol acyltransferase. In the latter case, its function consists in mediate the final step in TGAs synthesis using a fatty acyl-CoA and a DGA. This enzyme is vital in the storage and synthesis of lipids.

Based on Uniprot information, this enzyme is more likely to act as a wax ester synthase with (C12-C18)-CoA whereas with (C20)-CoA it acts as a DGAT.

Notes

Two transcriptional terminators (two biobricks BBa_B1006) were set at the end of the coding sequence. Also, to avoid RBS-CDS problems, the CDS prefix and suffix mentioned in this link (http://partsregistry.org/Assembly:RBS-CDS_issues#Standard_Assembly_and_the_RBS) were used.

Source

The amino sequence of this part was taken from Uniprot (wax-dgaT accesion Q8GGG1; http://www.uniprot.org/uniprot/Q8GGG1) and then codon optimization for R. opacus (avoiding restriction sites for the standard assembly method) was made using the free software GeneDesigner.

igem#experience
None
 
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K836002/1