Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Ricardo Alvarado
Date created: 2012-09-17 11:00:00
Date modified: 2015-05-08 01:13:32
lipA from B. cepacia (codon usage optimized for E. coli)
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K836003_sequence (Version 1) |
Description
This biobrick codifies for the protein lipA from Burkholderia cepacia (Pseudomonas cepacia) which catalyzes the hydrolysis of triglycerides (Triacylglycerol + H2O = diacylglycerol + a carboxylate). In the presence of certain alcohols, it catalyzes the transesterification of TGAs to produce acyl esters and glycerol.It has only one PTM (Post-Translational Modification) which consists in a sulfide bond between 234 and 314 aa. Calcium serves as a cofactor for this enzyme (1 ion per subunit). The native signal peptide was not modified.
Notes
The sequence of a transcriptional terminator (biobrick BBa_B1006) was set at the end of the coding sequence. Also, to avoid RBS-CDS problems, the CDS prefix and suffix mentioned in this link (http://partsregistry.org/Assembly:RBS-CDS_issues#Standard_Assembly_and_the_RBS) were used (not in the sequence presented here).Source
The amino sequence of this part was taken from Uniprot (lipA accesion P22088; http://www.uniprot.org/uniprot/P22088) and then codon optimization for E. coli (avoiding restriction sites for the standard assembly method) was made using the free software GeneDesigner.| Access | Instance | Definition |
| public public | BBa_K836000 BBa_B1006 | LipA BBa_B1006 |