Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Ricardo Alvarado
Date created: 2012-09-19 11:00:00
Date modified: 2015-05-08 01:13:32
Lysis inhibitor from Enterobacteria phage lambda (codon usage optimized for R. opacus)
| Types | DnaRegion |
| Roles | engineered_region Translational_Unit |
| Sequences | BBa_K836004_sequence (Version 1) |
Description
This protein is the one in charge to avoid the formation of pores in the inner membrane of the bacterial cell. It has been optimized for expression in R. opacus. A weak RBS for Gram positive bacteria was used (http://partsregistry.org/Part:BBa_K090506:Design)in order to obtain low concentration of the protein.Notes
We wanted a low concentration of this protein since medium to large concentrations could affect or completely inhibit the lysis device. Basal but constitutive expression of this protein is needed to avoid the formation of pores in the design lysis device.Source
The amino sequence was obtained from Uniprot (http://www.uniprot.org/uniprot/P03705#section_seq lysis inhibitor isoform). The codon optimization for R. opacus (avoiding standard restriction sites EcoRI, PstI, XbaI and SpeI) was made using the free software GeneDesigner from DNA 2.0.| Sequence Annotation | Location | Component / Role(s) |
| BBa_K090506 Scar Lambda antiholin | 1,11 12,17 18,338 | ribosome_entry_site feature/rbs feature/misc sequence_feature feature/cds CDS |