| Types | DnaRegion
|
| Roles | promoter
Regulatory
|
| Sequences | BBa_K837001_sequence (Version 1)
|
Description
The part includes an RNAT, which is a temperature-sensing sequence, in front of the reporter eGFP. As the environmental temperature changes below 30℃, the RNAT folds into a series of different secondary tructures and block ribosomes??? access to the mRNA thus hinder translation of eGFP.
Notes
The SD sequence that benefits the bond between the RNA and the ribosome is designed in front of the eGFP sequence.
Source
The RNAT sequence is designed by us, using computational method. The eGFP gene is from the iGEM part,BBa_I714891.
