BBa_K842001

BBa_K842001 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K842001

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Sean P. Curran, Percy Genyk, Ellen Park, Rebecca, Gao, Stephan Genyk, Megan Bernstein, Rachel Kohan, Eric Siryj, Luke Quinto

Date created: 2012-10-01 11:00:00

Date modified: 2015-05-08 01:13:33

flhD

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K842001_sequence (Version 1)

Description

flhD along with flhC forms the master regulation operon for the synthesis of flagella in E. coli. They are responsible for the downstream transcription of the class 2 flagella genes. flhD is directly induced by several separate systems, among these is the two-component quorum sensing response system, qseB and qseC.

Used for reconstitution of flagella apparatus in E. coli B strains.

Cloning and Uses
This part was generated via PCR from the E. coli strain DH5α and cloned into the vector pSB1C3. FlhD is to be used in conjunction with flhC in order to form the transcriptional operon for the production of flagella. This part can be used either to generate a flagella in an E. coli strain in which they are not already present, or to promote further formation of pre-existing flagella.

Notes

none

Source

Flagellar transcriptional regulator FlhD ??? Escherichia coli (strain K12). (n.d.). UniProt. Retrieved October 1, 2012, from http://www.uniprot.org/uniprot/P0A8S9

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
flhD	1,360	CDS, feature/cds

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K842001/1

Member of these Collections

• iGEM Parts Registry