BBa_K842010

BBa_K842010 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K842010

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Sean P. Curran, Percy Genyk, Ellen Park, Rebecca, Gao, Stephan Genyk, Megan Bernstein, Rachel Kohan, Eric Siryj, Luke Quinto

Date created: 2012-10-01 11:00:00

Date modified: 2015-05-08 01:13:33

cheZ

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K842010_sequence (Version 1)

Description

CheZ is a regulatory protein that constantly dephosphorylates cheY that is attached to a flagellum. The incessant dephosphorylation is what causes bacteria to run, stop, and tumble when in an environment that has a concentration gradient and remain responsive to any changes in chemical concentration.

Used for researcher control of signaling mechanisms that influence flagella function.

Cloning and Uses
This part was generated via PCR from the E. coli strain DH5α and cloned into the vector pSB1C3. It is to be used with other chemotaxis genes to form a complete regulatory pathway that controls the response system in MCP and the direction of rotation in flagella.

Notes

none

Source

Protein phosphatase CheZ ??? Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720). (n.d.). UniProt. Retrieved October 1, 2012, from http://www.uniprot.org/uniprot/P07800

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
cheZ	1,645	feature/cds, CDS

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K842010/1

Member of these Collections

• iGEM Parts Registry