BBa_K844008

BBa_K844008 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K844008
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Andrea Halling
Date created: 2012-10-01 11:00:00
Date modified: 2016-02-10 11:28:32

Spider Silk 1x Subunit "B" (Balanced tRNA codon optimized) with Met (ATG) added



Types
DnaRegion

Roles
Coding

CDS

Sequences BBa_K844008_sequence (Version 1)

Description

Spider silk subunit optimized to use a reduced set of tRNA codons (1 or 2 codons per amino acid); contains two elasticity domains and one strength domain, also contains 5??? Met (ATG) codon.

Notes

none

Source

Created using mutagenesis PCR to add upstream Met (ATG) to part BBa_K844004

Sequence Annotation Location Component / Role(s)
Start
Beta-helix
Elasticity Domain
Beta-spiral
Beta-spiral
Beta-spiral
Beta-spiral
Beta-helix
Elasticity Domain
Beta-spiral
Beta-sprial
Beta-spiral
Beta-spiral
Linker Domain
Strength Domain
1,3
4,12
4,87
13,27
34,48
49,63
72,87
88,97
88,171
98,113
117,132
133,147
156,171
172,188
189,207
start_codon feature/start
sequence_feature feature/misc
sequence_feature feature/misc
sequence_feature feature/misc
sequence_feature feature/misc
sequence_feature feature/misc
feature/misc sequence_feature
feature/misc sequence_feature
sequence_feature feature/misc
feature/misc sequence_feature
sequence_feature feature/misc
sequence_feature feature/misc
sequence_feature feature/misc
sequence_feature feature/misc
feature/misc sequence_feature
igem#experience
None
 
igem#partStatus
Released HQ 2013
igem#sampleStatus
In stock
igem#status
Available
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K844008/1