Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Kathleen Miller
Date created: 2012-10-02 11:00:00
Date modified: 2015-05-08 01:13:33
Enhanced tRNA Promoter for E. coli
| Types | DnaRegion |
| Roles | promoter Regulatory |
| Sequences | BBa_K844010_sequence (Version 1) |
Description
This part is a mutated version of the E. coli leuV tRNA promoter. This part is based on the ZH14 sequence from Bauer et al. (1993). This promoter has approximately normal growth-rate dependent regulation but 12.0x normal promoter strength in rich medium.It is important to use a tRNA promoter to express tRNA parts, as other promoters will recruit RNA polymerase II, which only synthesizes mRNA. The tRNA promoter will recruit RNA polymerase III, which is the correct polymerase for synthesizing tRNAs.
Notes
noneSource
Based on the ZH14 sequence from Bauer et al. (1993), which is a mutated version of leuV tRNA promoter from E. coli.| Sequence Annotation | Location | Component / Role(s) |
| tRNA promoter for E. coli | 1,40 | feature/promoter promoter |