Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Kathleen Miller
Date created: 2012-10-02 11:00:00
Date modified: 2015-05-08 01:13:33
tRNA Terminator for E. coli
| Types | DnaRegion |
| Roles | terminator Terminator |
| Sequences | BBa_K844011_sequence (Version 1) |
Description
This tRNA terminator is based around the downstream sequence of the E. coli Ala(GCC) tRNA gene. It contains the region of 3-6 T???s that is characteristic of tRNA terminators and an additional 10 bp of native sequence. This additional region of native sequence is to ensure RNA termination and to act as a small spacer region between tRNA genes.It is important to use tRNA specific terminators when expressing tRNA genes, as normal rho-dependent and rho-independent terminators rely on additional RNA sequences after where the gene actually finishes to terminate, and when extra sequences are included on tRNA molecules, their function can be severely affected.
Notes
noneSource
E. coli genomic DNA downstream of the Ala(GCC) tRNA gene.| Sequence Annotation | Location | Component / Role(s) |
| tRNA terminator Spacer | 1,5 6,15 | sequence_feature feature/misc feature/misc sequence_feature |