Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Andrea Halling
Date created: 2012-10-02 11:00:00
Date modified: 2015-05-08 01:13:33
tRNA expression cassette for spider silk "F" proteins
| Types | DnaRegion |
| Roles | Generator engineered_region |
| Sequences | BBa_K844012_sequence (Version 1) |
Description
Description goes hereNotes
When working with tRNA expression systems, you cannot have scar sites between the promoter and the gene, or between the gene and the terminator. As such, the entire sequence was synthesized without scars. At minimum, no scars can be internal to an individual tRNA expression unit, but can exist between different complete expression units.Source
This part was chemically synthesized but based upon E. coli genomic DNA sequences, and BioBrick parts BBa_K844010 and BBa_K844011.| Sequence Annotation | Location | Component / Role(s) |
| BBa_K844010 GlyGCC (GGT codon) tRNA gene from E. coli BBa_K844011 BBa_K844010 GlnTTG (CAA codon) tRNA gene from E. coli BBa_K844011 BBa_K844010 BBa_K844011 BBa_K844010 ProGGG (CCT codon) tRNA gene from E. coli BBa_K844010 AlaTGC (GCA codon) tRNA gene from E. coli BBa_K844011 SerGCT (AGT codon) tRNA gene from E. coli BBa_K844011 BBa_K844010 TyrGTA (TAT codon) tRNA gene from E. coli BBa_K844011 | 1,40 41,116 117,132 133,172 173,247 248,262 263,302 380,394 395,434 303,379 551,565 435,510 511,550 566,658 659,673 674,713 714,798 799,812 | feature/promoter promoter CDS feature/cds feature/misc sequence_feature promoter feature/promoter CDS feature/cds feature/misc sequence_feature promoter feature/promoter feature/misc sequence_feature feature/promoter promoter feature/cds CDS feature/promoter promoter CDS feature/cds sequence_feature feature/misc feature/cds CDS sequence_feature feature/misc feature/promoter promoter feature/cds CDS sequence_feature feature/misc |