Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Ryan Putman
Date created: 2012-10-02 11:00:00
Date modified: 2015-05-08 01:13:33
tRNA expression cassette for spider silk "B" proteins
| Types | DnaRegion |
| Roles | Generator engineered_region |
| Sequences | BBa_K844013_sequence (Version 1) |
Description
Description goes hereNotes
When working with tRNA expression systems, you cannot have scar sites between the promoter and the gene, or between the gene and the terminator. As such, the entire sequence was synthesized without scars. At minimum, no scars can be internal to an individual tRNA expression unit, but can exist between different complete expression units.Source
This part was chemically synthesized but based upon E. coli genomic DNA sequences, and BioBrick parts BBa_K844010 and BBa_K844011.| Sequence Annotation | Location | Component / Role(s) |
| BBa_K844010 GlyTCC (GGA codon) tRNA from E. coli BBa_K844010 BBa_K844011 ProTGG (CCA codon) tRNA from E. coli BBa_K844011 BBa_K844010 SerGGA (TCT codon) tRNA from E. coli BBa_K844011 BBa_K844010 ThrGGT (ACT codon) tRNA from E. coli BBa_K844011 BBa_K84401 ArgTCT (AGA codon) tRNA from E. coli BBa_K844011 | 1,40 41,115 131,170 116,130 171,247 248,262 263,302 303,390 391,405 406,445 446,521 522,536 537,576 577,653 654,668 | promoter feature/promoter CDS feature/cds feature/promoter promoter sequence_feature feature/misc feature/cds CDS sequence_feature feature/misc promoter feature/promoter CDS feature/cds sequence_feature feature/misc feature/promoter promoter feature/cds CDS feature/misc sequence_feature feature/promoter promoter CDS feature/cds sequence_feature feature/misc |