Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Gr??gory Hansen
Date created: 2012-09-25 11:00:00
Date modified: 2015-05-08 01:13:33
New paraBAD part
| Types | DnaRegion |
| Roles | Regulatory promoter |
| Sequences | BBa_K845001_sequence (Version 1) |
Description
iGEM 2012 Grenoble Team proposes a new design and application to the pBAD promoter(paraBAD).In order to create a biological AND gate the 2012 Grenoble team worked on the cAMP-CRP complex and arabinose inductible promoter.
Notes
Our pBAD promoter has a longer sequence compared to the ones already registered and is composed of six regulation sites.417 bp from the promoter to the ATG of araB plus the 82 first bp of the araB gene.
Source
pBAD is a natural promoter in E. Coli and is part of its genomic sequence. It regulates the production of three proteins (AraA, AraB and AraD) which form the arabinose operon. Their production enables the use of arabinose as a carbon source.It has six regulation sites, five of them are devoted to AraC fixation (three are repressing, one has a dual activity and one is an activator); the last one is a CRP binding site (positive activity).
This promoter is activated when both activated CRP and AraC are bind to it.
| Sequence Annotation | Location | Component / Role(s) |
| paraBAD AraC activator AraC dual activity (inhibitor/activator) CRP activator AraC inhibitor AraC inhibitor AraC inhibitor AraB 82 first bp | 1,499 367,383 346,362 314,335 293,309 272,288 135,151 418,499 | feature/promoter promoter non_covalent_binding_site feature/binding feature/binding non_covalent_binding_site feature/binding non_covalent_binding_site non_covalent_binding_site feature/binding non_covalent_binding_site feature/binding feature/binding non_covalent_binding_site sequence_feature feature/misc |