Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Antoine Ribeiro
Date created: 2012-09-25 11:00:00
Date modified: 2015-05-08 01:13:34
luxI-lacI-mCherry under LsrA/cI/tetO hybrid promoter
| Types | DnaRegion |
| Roles | Composite engineered_region |
Description
The LsrA promoter is repressed by LsrR protein (BBa_K848000). Repression is inhibited by the presence of AI-2 signal molecule phosphorilated by LsrK protein (BBa_K848001) . The cI promoter is repressed by the cI repressor (BBa_C0051)The TetO promoter is repressed by TetR. TetR repression is inhibited by the addition of tetracycline.
This part produces LuxI and LacI signal molecules with a mCherry reporter. LuxI is a quorum sensing protein that converts SAM into acyl-homoserine lactone (AHL) thus acting a pLuxR promoter activator when bound to LuxR protein (BBa_K329007)
LacI binds to the pLac regulator (BBa_R0010) and PLlac01 hybrid regulator (BBa_R0011) and inhibits transcription. IPTG binds to LacI and inhibits its operation, therefore promoting transcription.