Types | DnaRegion
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Roles | Composite
engineered_region
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Description
The LsrA promoter is repressed by LsrR protein (BBa_K848000). Repression is inhibited by the presence of AI-2 signal molecule phosphorilated by LsrK protein (BBa_K848001) . The cI promoter is repressed by the cI repressor (BBa_C0051)
The TetO promoter is repressed by TetR. TetR repression is inhibited by the addition of tetracycline.
This part produces LuxI and LacI signal molecules with a mCherry reporter. LuxI is a quorum sensing protein that converts SAM into acyl-homoserine lactone (AHL) thus acting a pLuxR promoter activator when bound to LuxR protein (BBa_K329007)
LacI binds to the pLac regulator (BBa_R0010) and PLlac01 hybrid regulator (BBa_R0011) and inhibits transcription. IPTG binds to LacI and inhibits its operation, therefore promoting transcription.
Notes
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Source
BBa_S03816