Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Ma Tsz Shan
Date created: 2012-10-01 11:00:00
Date modified: 2015-05-08 01:13:34
pvdQ gene with a silent mutation at 1494 bp to remove the internal PstI site
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K855006_sequence (Version 1) |
Description
pvdQ gene codes for an acylase that can degrade long-chain N-acylhomoserine lactones (AHLs), e.g. 3-oxo-C12-HSL. A site-directed mutagenesis at 1494 bp is introduced to the glycine codon, CAG > CAA, to remove the internal illegal PstI site. After the mutagenesis, the biobrick will be compatible with the standard assembly and the pvdQ gene expressed will still be functional.Notes
A His tag is added to the C-terminal of the coding sequence to help detect the expressed protein.Source
pseudomonas aeruginosa PAO1| Sequence Annotation | Location | Component / Role(s) |
| signal peptide pvdQ gene silent mutation "G > A" to remove the PstI site stop his tag | 1,69 70,2286 1494,1494 2305,2307 2287,2304 | sequence_feature feature/misc CDS feature/protein feature/mutation sequence_alteration stop_codon feature/stop tag feature/tag |