BBa_K861003

BBa_K861003 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K861003

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Kuanwei Sheng

Date created: 2012-09-20 11:00:00

Date modified: 2015-05-08 01:13:35

PfadR regulated FadL

Details

• Types: DnaRegion
• Roles: engineered_region; Generator
• Sequences: BBa_K861003_sequence (Version 1)

Description

Long-chain fatty acids are transported across the cell membrane via a transport/acyl-activation mechanism involving an outer membrane protein, FadL(BBa_K861500). The fadL gene codes for a 43,000-dalton membrane protein (FLP) which has been implicated as being essential for LCFA transport. When expressed, FadL can transport the long-chain fatty acids into the bacterium. We assebled FadL with PfadR (BBa_K861060) hoping it will only be overexpressed when fatty acids concentration is high.

Notes

None

Source

FadL gene is cloned from E.coli K12 DH5α

Components

Access	Instance	Definition
public	BBa_K861060	PfadR
public	BBa_K861001	BBa_K861001

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K861060	1,76	PfadR
BBa_K861001	85,1549	BBa_K861001

Other Properties

• igem#experience: Works
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K861003/1

Member of these Collections

• iGEM Parts Registry