Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Xian Xia
Date created: 2012-09-21 11:00:00
Date modified: 2015-05-08 01:13:36
Indirect regulatory device, activated at high glucose concentration
| Types | DnaRegion |
| Roles | Device engineered_region |
| Sequences | BBa_K861169_sequence (Version 1) |
Description
This is an indirect regulatory devices activated at high glucose concentration through an intermediate protein cI of lambda phage. In this pathway, protein cI will be expressed when CRP is activated at low glucose concentration. cI will therefore repress the expression of gene downstream the promoter BBa_R0051 heavily. On the contrary, when glucose concentration is high, the promoter will be derepressed.There was no promoter existing in the nature which is directly activated by glucose. In this device, RFP was designed as a reporter. RFP can be placed by an arbitrary protein that needs to be activated by glucose.
Notes
NoneSource
From the registry| Access | Instance | Definition |
| public public public public public | BBa_K118011 BBa_B0034 BBa_C0051 BBa_B0024 BBa_I763007 | BBa_K118011 BBa_B0034 cI lam BBa_B0024 pLambda+RF |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K118011 BBa_B0034 BBa_C0051 BBa_B0024 BBa_I763007 | 1,131 140,151 158,932 941,1035 1044,1961 | BBa_K118011 BBa_B0034 cI lam BBa_B0024 pLambda+RF |