Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Kuanwei Sheng, Xian Xia
Date created: 2012-09-07 11:00:00
Date modified: 2015-05-08 01:13:36
mRFP expressed after Pcar
| Types | DnaRegion |
| Roles | engineered_region Reporter |
| Sequences | BBa_K861176_sequence (Version 1) |
Description
PII is a promoter designed for the Eschaerichia coli . It includes the CRP-binding site and the RNA polymerase-binding site which have a overlap of several base pairs.Therefore due to the steric hindrance between CRP and RNA polymerase,gene downstream of the promoter will be repressed at high concentration of CRP.In the cells ,low glucose concentration results in increased activity by adenylate cyclase. cAMP binds to the cAMP receptor protein, which, in its bound form, is able to bind tightly to the specific DNA site in the promoter and repress the gene downstream.On the contrary,high glucose concentration will result in the expression of the promoter.This generator contains PII and a screening plasmid intermediate designed by Josh.Notes
NoneSource
Escherichia coli str. K-12 substr. MG1655| Access | Instance | Definition |
| public public | BBa_K861171 BBa_I13507 | Pcar BBa_I13507 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K861171 BBa_I13507 | 1,36 45,905 | Pcar BBa_I13507 |