precB

BBa_K862003 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K862003

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Charlotte Bunne, Mariam Harmouche, Stefan Holderbach, Anna Huhn and Jakob Kreft (in alphabetical order)

Date created: 2012-06-07 11:00:00

Date modified: 2015-05-08 01:13:36

precB

Details

• Types: DnaRegion
• Roles: promoter; Regulatory
• Sequences: BBa_K862003_sequence (Version 1)

Description

The recB promoter sequence was taken for the E. coli MG1655 genome sequence (http://ecoliwiki.net/colipedia/index.php/recB:Gene). We assumed the main promoter region to be located from -70 to -1 bp upstream the recB start codon. Therefore this sequence was synthesized and cloned on an oligo basis.

We will not submit the physical DNA of this part, but we provide the oligo-sequences you can use for synthesizing and cloning this part in front of any EcoRI/XbaI precut reporter part.

RecB_fw: aattcgcggccgcttctagagCCTGAAGGCTGGAAAGTGTGGGAGAACGTCAGCGCGTTGCAGCAAACAATGCCCCTGATGAGTGAAAAGAc
RecB_rev: ctaggTCTTTTCACTCATCAGGGGCATTGTTTGCTGCAACGCGCTGACGTTCTCCCACACTTTCCAGCCTTCAGGctctagaagcggccgcg

Notes

the sequence was ordered on a synthetic oligo basis and cloned in front of EcoRI/XbaI reporter parts.

Source

E. coli MG1655 genome: http://ecoliwiki.net/colipedia/index.php/recB:Gene
bp -70 to -1 before recB coding sequence.

Other Properties

• igem#experience: Works
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K862003/1

Member of these Collections

• //chassis/prokaryote
• //chassis/prokaryote/ecoli
• //function/sensor
• iGEM Parts Registry