CBDcex(T7)

BBa_K863103 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K863103
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Moritz M??ller
Date created: 2012-09-19 11:00:00
Date modified: 2015-06-17 01:19:26

Cellulose binding Domain from Cellulomonas Fimi with Reporter GFP



Types
DnaRegion

Roles
engineered_region

Reporter

Sequences BBa_K863103_sequence (Version 1)

Description

Cellulose binding Domain of C. Fimi Exoglucanase gene with T7, RBS linked with two amino acids (GS) to a GFP (Freiburg-Standard)

Notes

CBDcex fused to a his-tagged GFP in a Freiburg-manner

Source

Cloning-vector and BBa_K863121

Sequence Annotation Location Component / Role(s)
T7 promoter
BBa_K525998
BBa_K863102
B0034
RBS
ATG
Exoglucanase
BBa_K863101
Cellulose binding domain (CBDcex)
Exoglucanase
GS-Linker
Freiburg-Scar
BBa_E0040
BBa_K863121
His-Tag
AgeI
TAA
1,20
1,32
1,364
21,32
24,27
35,37
38,49
38,364
50,349
350,358
359,364
365,370
371,1084
371,1111
1085,1102
1103,1108
1109,1111
promoter feature/promoter
feature/BioBrick engineered_region
feature/BioBrick engineered_region
feature/BioBrick engineered_region
feature/rbs ribosome_entry_site
feature/start start_codon
conserved_region feature/conserved
engineered_region feature/BioBrick
CDS feature/protein
feature/conserved conserved_region
feature/cds CDS
sequence_feature feature/misc
engineered_region feature/BioBrick
engineered_region feature/BioBrick
tag feature/tag
sequence_feature feature/misc
stop_codon feature/stop
igem#experience
None
 
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K863103/1