CBDclos

BBa_K863111 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K863111
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Moritz M??ller
Date created: 2012-09-17 11:00:00
Date modified: 2015-06-17 01:18:55

Cellulose binding domain of C. cellulovorans cellulose binding protein gene (Freiburg-Standard)



Types
DnaRegion

Roles
polypeptide_domain

Protein_Domain

Sequences BBa_K863111_sequence (Version 1)

Description

Cellulose binding Domain of Clostridium cellulovorans cellulose binding protein gene (cbp A)
http://www.ncbi.nlm.nih.gov/nuccore/M73817

Notes

The sequence starts with the Freiburg-prefix (ATG+NgoMIV-restriction-site) followed by 12 Bases (4 AS) of the cellulose binding protein gene in front of the CBD; then 12 bases (4 amino acids) of the cellulose binding protein gene behind the CBD; after that the sequence continues with a short GS-Linker and ends with the Freiburg-suffix (AgeI-restirction-site+TAA).

Source

Cloning-Vector

Sequence Annotation Location Component / Role(s)
ATG
NgoMIV
Cellulose Binding Protein
TAA
AgeI
Cellulose binding domain (CBDclos)
Cellulose Binding Protein
GS-Linker
Siltent mutation (A original)
1,3
4,9
10,21
316,318
310,315
22,297
298,303
304,309
93,93
start_codon feature/start
feature/misc sequence_feature
conserved_region feature/conserved
stop_codon feature/stop
sequence_feature feature/misc
CDS feature/protein
feature/conserved conserved_region
feature/cds CDS
feature/s_mutation point_mutation
igem#experience
None
 
igem#sampleStatus
In stock
igem#status
Available
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K863111/1