Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Moritz M??ller
Date created: 2012-09-19 11:00:00
Date modified: 2015-06-17 01:20:18
Cellulose binding domain of C. cellulovorans with T7, RBS, GS-Linker (Freiburg-Standard)
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K863112_sequence (Version 1) |
Description
Cellulose binding domain of the Cellulomonas fimi ATCC 484 exoglucanase gene in Freiburg-standardNotes
Added the regulatory unit BBa_K525998 (T7+RBS) in front of the CBD and a short GS-Linker behind the domain and used Freiburg-Standard to be able to fuse proteins to the domain.Source
Cloning-vector| Sequence Annotation | Location | Component / Role(s) |
| T7 promoter BBa_K525998 B0034 RBS BBa_K863111 ATG Cellulose Binding Protein Cellulose binding domain (CBDclos) Cellulose Binding Protein GS-Linker AgeI TAA silent mutation (A original) | 1,20 1,32 21,32 24,27 35,337 35,38 35,40 41,316 317,322 323,328 329,334 335,337 112,112 | promoter feature/promoter feature/BioBrick engineered_region feature/BioBrick engineered_region feature/rbs ribosome_entry_site feature/BioBrick engineered_region feature/start start_codon conserved_region feature/conserved CDS feature/protein conserved_region feature/conserved feature/cds CDS feature/misc sequence_feature feature/stop stop_codon point_mutation feature/s_mutation |