Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Moritz M??ller
Date created: 2012-09-19 11:00:00
Date modified: 2015-06-17 01:20:29
Cellulose binding Domain of C. cellulovorans cellulose binding protein with Reporter GFP
| Types | DnaRegion |
| Roles | engineered_region Reporter |
| Sequences | BBa_K863113_sequence (Version 1) |
Description
Cellulose binding Domain from Clostridium cellulovorans cellulose binding protein gene with T7, RBS linked with two amino acids (GS) to a GFP with His-tag.Notes
CBDclos fused to a his-tagged GFP in a Freiburg-manner| Sequence Annotation | Location | Component / Role(s) |
| BBa_K863112 BBa_K525998 T7 promoter B0034 RBS ATG Cellulose Binding Protein BBa_K863111 Cellulose binding domain (CBDclos) Cellulose Binding Protein GS-Linker Freiburg-Scar BBa_K863121 BBa_E0040 His-Tag AgeI TAA Silent mutation (A original) | 1,328 1,32 1,20 21,32 24,27 35,38 35,40 35,328 41,316 317,322 323,328 329,334 335,1075 335,1048 1049,1066 1068,1072 1073,1075 112,112 | feature/BioBrick engineered_region engineered_region feature/BioBrick feature/promoter promoter engineered_region feature/BioBrick ribosome_entry_site feature/rbs feature/start start_codon feature/conserved conserved_region engineered_region feature/BioBrick feature/protein CDS conserved_region feature/conserved CDS feature/cds sequence_feature feature/misc engineered_region feature/BioBrick feature/BioBrick engineered_region tag feature/tag feature/misc sequence_feature feature/stop stop_codon feature/s_mutation point_mutation |