Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Erik Lundin
Date created: 2012-09-23 11:00:00
Date modified: 2015-05-08 01:13:37
Low copy BioBrick standard vector with LacI
| Types | DnaRegion |
| Roles | Plasmid_Backbone plasmid_vector |
| Sequences | BBa_K864009_sequence (Version 1) |
Description
pSB4A15Iq is a BioBrick standard vector with low copy pSC101 replication origin (~5 copies per cell) and ampicillin antibiotic resistance marker, especially usable for Lambda Red recombineering in E coli. The backbone sequence is based on pSB3T5, but the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22.This vector carries a LacIq cassette on the backbone for tight repression of any lac promoter cloned into the cloning site.
Notes
The resistance cassette is terminated by a Lux bidirectional terminator. Adjacent to this terminator there is a designed primer binding site to where the forward primer for lambda red can be designed to bind to no matter what the insert in the cloning site is or what the resistance is.The resistance cassette is flanked by SalI and SacI restriction sites for easy switching of resistance in the backbone.
Source
Based on [[Part:BBa_K864001|BBa_K864001]]| Sequence Annotation | Location | Component / Role(s) |
| pSC101 ori Promoter PlacIq promoter lacI cmR RBS -35 -35 Lux terminator P22 late terminator T3 terminator tonB terminator rrnC -10 -10 | 190,2405 4415,4441 2439,2494 2521,3603 3686,4348 2509,2514 881,886 2441,2446 3634,3679 4461,4501 4522,4599 47,115 136,175 903,908 2464,2469 | feature/misc sequence_feature feature/promoter promoter promoter feature/promoter feature/cds CDS CDS feature/cds ribosome_entry_site feature/rbs feature/misc sequence_feature feature/misc sequence_feature feature/stop stop_codon feature/stop stop_codon feature/misc sequence_feature sequence_feature feature/misc sequence_feature feature/misc sequence_feature feature/misc feature/misc sequence_feature |