Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Erik Lundin
Date created: 2012-09-24 11:00:00
Date modified: 2015-05-08 01:13:37
pSC101 low copy origin of replication
| Types | DnaRegion |
| Roles | sequence_feature DNA |
| Sequences | BBa_K864050_sequence (Version 1) |
Description
BBa_K864050 is a pSC101 origin of replication and is found in the pSB4*15 series of BioBrick vectors. This ori is an improvement of the ori found in all pSB4*5 plasmids, [[Part:BBa_I50042|BBa_I50042]]. [[Part:BBa_I50042|BBa_I50042]] has been shown to have a higher copy number than expected.Notes
This ori was created by cloning pSC101ts from pSIM5 and making a V56A mutation in repA.Source
pSIM5| Sequence Annotation | Location | Component / Role(s) |
| par region pSC101 ori Repeat structure Repeat structure AT rich region Tandem repeat Repeated sequence Tandem repeat Repeated sequence Tandem repeat Repeated sequence Repeat "4" Repeated sequence A>T mutation to remove SpeI Hairpin structure -35 signal Repeat "5" Repeated sequence repA Hairpin structure Ins(GGC) | 1,368 1,2216 190,204 232,247 499,581 578,599 582,589 601,620 603,610 631,652 635,642 673,691 676,683 678,678 683,761 692,697 702,723 712,719 750,1700 1608,1682 2141,2143 | sequence_feature feature/misc sequence_feature feature/misc feature/misc sequence_feature feature/misc sequence_feature sequence_feature feature/misc feature/misc sequence_feature sequence_feature feature/misc sequence_feature feature/misc sequence_feature feature/misc sequence_feature feature/misc feature/misc sequence_feature feature/misc sequence_feature feature/misc sequence_feature sequence_alteration feature/mutation feature/stem_loop stem_loop feature/promoter promoter sequence_feature feature/misc sequence_feature feature/misc feature/cds CDS feature/stem_loop stem_loop feature/mutation sequence_alteration |