SYFP2

BBa_K864100 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K864100
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Erik Gullberg
Date created: 2012-09-23 11:00:00
Date modified: 2016-01-21 02:29:24

Super Yellow Fluorescent Protein 2 (SYFP2)



Types
DnaRegion

Roles
Coding

CDS

Sequences BBa_K864100_sequence (Version 1)

Description

This part codes for the bright yellow fluorescent protein SYFP2. SYFP2 is a GFP based monomeric protein with a narrow fluorescence emission spectrum with a maximum at 515 nm. Bacteria expressing SYFP2 are reported to be 12 times brighter than those expressing EYFP(Q69K) and almost 2-fold brighter than bacteria expressing Venus (Ref. 10.1021/bi0516273). Codon optimized for expression in E coli by DNA 2.0. Mutations compared to wtGFP amino acid sequence (GenBank Accession number M62653) are F46L F64L S65G S72A M153T V163A S175G T203Y A206K.

Notes

The sequence has been codon optimized for expression in E coli by DNA 2.0.

Source

Amino acid sequence taken from the article by Kremers et al 2006.

Sequence Annotation Location Component / Role(s)
SYFP2
1,717
CDS feature/cds
igem#experience
Works
 
igem#partStatus
Released HQ 2013
igem#sampleStatus
In stock
igem#status
Available
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K864100/1