| Types | DnaRegion
|
| Roles | Coding
CDS
|
| Sequences | BBa_K864100_sequence (Version 1)
|
Description
This part codes for the bright yellow fluorescent protein SYFP2. SYFP2 is a GFP based monomeric protein with a narrow fluorescence emission spectrum with a maximum at 515 nm. Bacteria expressing SYFP2 are reported to be 12 times brighter than those expressing EYFP(Q69K) and almost 2-fold brighter than bacteria expressing Venus (Ref. 10.1021/bi0516273). Codon optimized for expression in E coli by DNA 2.0. Mutations compared to wtGFP amino acid sequence (GenBank Accession number M62653) are F46L F64L S65G S72A M153T V163A S175G T203Y A206K.
Notes
The sequence has been codon optimized for expression in E coli by DNA 2.0.
Source
Amino acid sequence taken from the article by Kremers et al 2006.
