BBa_K864204

BBa_K864204 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K864204
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Erik Lundin
Date created: 2012-09-24 11:00:00
Date modified: 2015-05-08 01:13:37

OmpF



    • Details

    Types
    DnaRegion

    Roles
    CDS

    Coding

    Sequences BBa_K864204_sequence (Version 1)

    Description

    OmpF

    Notes

    Illegal PstI restriction site removed by silent mutations T>A at position 66 and A>T at position 69 using primers listed below (annealing sequences are underlined)

    ompF_rsr_F
    CTGAAATCTATAACAAAGATGGCAA

    ompF_rsr_R
    CTGCGTTTGCAGTACCTGCT

    Source

    Amplified from MG1655 (accession NC_000913) chromosome using primers listed below (annealing sequences are underlined)

    ompF_BB_F
    TTCGAATTCGCGGCCGCTTCTAGATGATGAAGCGCAATATTC

    ompF_BB_R
    CGGCCGCTACTAGTATTATTAGAACTGGTAAACGATACC

    Sequence Annotation Location Component / Role(s)
    ompF
    T>A
    A>T
    		1,1092
    66,66
    69,69
    		CDS feature/cds
    point_mutation feature/s_mutation
    point_mutation feature/s_mutation

    igem#partStatus
    Released HQ 2013
    igem#sampleStatus
    In stock
    igem#status
    Available
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K864204/1
     

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