Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Hampus Elofsson, Sabri Jamal, Lisa Branzell
Date created: 2012-09-24 11:00:00
Date modified: 2015-05-08 01:13:37
Small RNA spot42
| Types | DnaRegion |
| Roles | RNA mature_transcript_region |
| Sequences | BBa_K864440_sequence (Version 1) |
Description
The natural smallRNA spot42 taken from the E-coli MG1655. SmallRNA is belived to interact in many metabolic pathways. The spot42 scaffold interacts with HFQ-proteins. It is in many cases shown to bind into the rbs area of the mRNA and thereby blocking translation initiation. ( V.Sharma, A.Yamamura Y.Yokobayashi, 2011 )We wanted to use this sRNA for gene downregulation on translational level, in this case block the ribosome from binding to the rbs area.
You can use this natural scaffold to make your own sRNA, inhibiting translation on a gene of your choice.
The sRNA is coupled to a strong promotor, J23101
Notes
It can be a bit tricky to do mutagenesis on it, like we did. We wanted to add a a random antisense area with primers.You cannot have any scars between the promotor and sRNA so we added our promotor with mutagenesis.
Source
It was biobricked from E-coli MG1655| Sequence Annotation | Location | Component / Role(s) |
| J23101 Spot42_Antisense Spot42_Scaffold | 1,35 36,91 92,144 | feature/promoter promoter sequence_feature feature/misc sequence_feature feature/misc |