Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Joel ??s
Date created: 2012-09-24 11:00:00
Date modified: 2015-05-08 01:13:37
RFP-Linker-SYFP2
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K864444_sequence (Version 1) |
Description
Use this part to insert any 5?????TR of your gene of interest to screen for downregulation of your gene with small RNA. PCR amplify your 5???UTR with EcoRI and BamHI and clone into any backbone carrying this part. Digest whis part with EcoRI and BamHI and as RFP is replaced, this part functions as a red/white screening system. This allows you to create a reporter system with a fluorescent marker for screening for functional small RNAs.SYFP2
When screening for working small RNAs, it's recommended to include the first 15 codons of the gene of interest.
Notes
-Source
Constructed from SYFP2| Sequence Annotation | Location | Component / Role(s) |
| RFP J04450 Plac B0034 RFP B0012 B0010 Linker BamHI SYFP2 | 1,1068 1,200 209,220 227,681 1029,1069 941,1020 1076,1105 1070,1075 1106,1825 | feature/misc sequence_feature feature/promoter promoter ribosome_entry_site feature/rbs CDS feature/cds feature/stop stop_codon stop_codon feature/stop sequence_feature feature/misc feature/misc sequence_feature CDS feature/cds |