BBa_K864446

BBa_K864446 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K864446

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Arvid Hed??n Gynn??, Sabri Jamal, Lisa Branzell, Hampus Elofsson

Date created: 2012-09-28 11:00:00

Date modified: 2015-05-08 01:13:37

Spot42 randomized reverse primer

Details

• Types: DnaRegion
• Roles: primer; Primer
• Sequences: BBa_K864446_sequence (Version 1)

Description

This part is a component of the Uppsala University iGEM 2012 modular small RNA screening system.

This primer is used to create a random sRNA library, together with K864445, by randomizing the mRNA-binding region of the spot42 small RNA (K864444). This library can be used to screen for synthetic silencing sRNA:s.

Note

Due to technical limitations, the correct sequence can not be entered into the registry. The entered sequence should be preceded by 20 N:s, to denote the randomized region, and thus read:

NNNNNNNNNNNNNNNNNNNNGCTAGCATAATACCTAGGAC

Notes

The melting temperature has been designed to match K864445.

Source

Own design.

Other Properties

• igem#experience: Works
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K864446/1

Member of these Collections

• iGEM Parts Registry