Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Michael Boelker
Date created: 2012-09-24 11:00:00
Date modified: 2015-05-08 01:13:37
CFP Fusion brick
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K866000_sequence (Version 1) |
Description
This is a coding sequence for the Cyan Fluorescent Protein. It is based on the biobrick http://partsregistry.org/Part:BBa_E0020, yet lags the bases "TG" from it's start codon, allowing direct fusion to other protein coding sequences for in-frame translation.Notes
Two nucleotides (T and G) were removed from the CFP-start codon via site-directed mutagenesis using the following primer:{| border=1 align=center
! Primer name
! Primer
! Length
! G/C content
! Tm
|-
| MS 16
| 5'-GCGAATTCGCGGCCGCTTCTAGAGTGAGCAAGGGCGAGGAGCT-3'
| 43 bp
| 62.7 %
| 90.3 ??C
|-
| MS 17
| 5'-GCCTGCAGCGGCCGCTACTAGTATTATTACTTGTACAGCTCGT-3'
| 43 bp
| 51.1 %
| 80.5 ??C
|}
Source
This part was designed from http://partsregistry.org/Part:BBa_E0020 with a deletion of "TG" in the start codon.| Sequence Annotation | Location | Component / Role(s) |
| Deletion of nucleotides T and G CFP-Fusion | 1,1 1,719 | point_mutation feature/s_mutation CDS feature/protein |