Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Marija Drikic, Giorgio Gargari
Date created: 2012-09-22 11:00:00
Date modified: 2015-05-08 01:13:38
T5LacO-PelB-scFv (scFv 54.6 antinorovirus)-6HIS-TT
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K875006_sequence (Version 1) |
Description
This construct is designed for the expression of secreted, already descibed engeneered antinorovirus (NoV) monoclonal antibody (mAb 54.6).The antibody is expressed in a single chain fragment variable (scFv) format containing light (VL) and heavy (VH) variable domains separeted by a flexible peptide linker. It has already been reported that the scFv 54.6 binds a native recombinant NoV particles (VLPs) and inhibits VLP interaction with cells. PelB is a leader sequence often used to produce secreted proteins.
The construct consistes of T5 Lac Operator (Bba_K875002), ribosomal binding site, PelB-scFv 54.6 antinorovirus, HIstidine tag (6HIS), Terminator (B0015).
Notes
This coding sequence was optimized for expression into the prokaryotic cells. It has a Histidine tag.Source
Gene synthesis.Reference:
???Recombinant norovirus-specific scFv inhibit virus-like particle binding to cellular ligands??? K. Ettayebi and M. E. Hardy. Published: 31 January 2008 in Virology Journal 2008, 5:21?