miniA

BBa_K877000 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K877000

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Alie Abele, David Pohlman

Date created: 2012-09-28 11:00:00

Date modified: 2015-05-08 01:13:39

Mini CipA coding region

Details

• Types: DnaRegion
• Roles: polypeptide_domain; Protein_Domain
• Sequences: BBa_K877000_sequence (Version 1)

Description

This part codes for a portion of the scaffoldin protein (cipA) from Clostridium thermocellum cellulosome. Two regions of cipA were ligated together to form this part: three type one cohesin regions and a type two dockerin region. A signaling peptide for export of the protein out of the cell is also coded in this part.

This part is unfinished; it contains 6 pst1 sites within its coding sequence.

Notes

The size of the original genomic sequence from Clostridium thermocellum was reduced from 7kb to ~1kb.

Source

This part was isolated from the cipA scaffoldin from Clostridium thermocellum. Two sets of primers were used - one to isolate the type 2 dockerin region (MiniA2) and another to isolate three type-one cohesin regions and a signaling peptide for export of the protein out of the cell (MiniA1). Therefore, this part does not have the cellulose binding region of C. thermocellum's cellulosome or 6 of the type one cohesin regions. This decreased the nucleotide sequence from 7kb to ~1kb.

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K877000/1

Member of these Collections

• iGEM Parts Registry