BBa_K877001

BBa_K877001 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K877001
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: David Pohlman, Alie Abele
Date created: 2012-09-30 11:00:00
Date modified: 2015-05-08 01:13:39

LPP-OmpA and CtCoh2 Combination



Types
DnaRegion

Roles
polypeptide_domain

Protein_Domain

Sequences BBa_K877001_sequence (Version 1)

Description

This part is a combination of the transmembrane anchoring protein, LPP-OmpA, from the 2008 Warsaw submission and the type 2 "Clostridium thermocellum" cohesin module.

Notes

The unstructuralized amino acid linker of LPP-OmpA in combination with a similar structure naturally occurring in the type 2 cohesin domain allowed the two domains to be combined without compromising functionality. The type cohesin amino acid linker was at the 5' end of the sequence while the LPP-OmpA amino acid linker was at the 3' end, because of this the sac1 restriction site added to the cohesin domain was added to the 5' end of its sequence.

Source

The type 2 cohesin domain of this sequence comes from "Clostridium thermocellum" genome sequence.

Sequence Annotation Location Component / Role(s)
NdeI
OmpA
ATG
linker
SacI
BBa_K103006
1,7
4,438
4,6
439,464
459,464
1,464
feature/misc sequence_feature
feature/cds CDS
feature/start start_codon
CDS feature/cds
sequence_feature feature/misc
feature/BioBrick engineered_region
igem#experience
None
 
igem#sampleStatus
In stock
igem#status
Available
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K877001/1