Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Josh Atkinson, Mike Ferguson, and Ben Parker
Date created: 2012-09-28 11:00:00
Date modified: 2015-05-08 01:13:40
Asymmetrically digestible reporter to assay the activity of DNA recombinases FimE K137007 and HbiF K
| Types | DnaRegion |
| Roles | Measurement engineered_region |
| Sequences | BBa_K880001_sequence (Version 1) |
Description
-IRR K137008 + Sequence with AgeI cut-site K165006 + IRL K137010(MI262)-Asymmetrically digestible reporter to assay the activity of FimE K137007 and HbiF K880000 recombinases.
K880001 is a utility for characterizing the functionality of fim based recombinases such as FimB, FimE, and HbiF and consists of K137008 and K137010 flanking a ???junk??? DNA sequence K165006 containing an off-center AgeI restriction site proximal to the BioBrick prefix; inversion of the fragment will cause the site to move towards the suffix.
Digestion of the unflipped reporter with EcoRI and AgeI will yield a ~70bp fragment when in a FimE-flippable orientation and a ~220bp fragment when in an HbiF flippable orientation. Alternatively, digestion with AgeI and PstI will yield fragments of roughly opposite orientation. The reporter in the registry sequence is FimE flippable.
Notes
N/ASource
Created from a smattering of DNA junk and HbiF/FimE recognition sequences| Access | Instance | Definition |
| public public public | BBa_K137008 BBa_K165006 BBa_K137010 | IRR BBa_K165006 fimE IRL |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K137008 BBa_K165006 BBa_K137010 | 1,35 44,295 304,338 | IRR BBa_K165006 fimE IRL |