Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Hugo de Vries
Date created: 2012-09-18 11:00:00
Date modified: 2015-05-08 01:13:40
CCMV_Negative coat protein
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K883160_sequence (Version 1) |
Description
This construct produces CCMV core antigen subunits. The subunits have been modified in such a way that they will fold into CCMV Virus-Like Particles that have a negative interior. This was done by replacing 6 Arginine and 3 Lysine residues at the N-Terminus with Glutamic acid residues.The full protocol for this construct can be found on the Wageningen_UR 2012 Wiki.
Notes
In nature, wild-type CCMV Virus Particles have a positive interior to which RNA molecules attach. To be able to load the CCMV VLPs with positively charged molecules, e.g. metals, we have re-designed the N-Terminus of the core antigen subunits to contain 9 negatively charged amino acids instead of 9 positively charged amino acids.Source
Modified from CCMV wild-type.| Sequence Annotation | Location | Component / Role(s) |
| CCMV with negative N-terminus | 1,578 | CDS feature/protein |