BBa_K883161

BBa_K883161 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K883161

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Hugo de Vries

Date created: 2012-09-23 11:00:00

Date modified: 2015-05-08 01:13:40

CCMV_Negative core antigen with IPTG induced promotor

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K883161_sequence (Version 1)

Description

This construct produces CCMV core antigen subunits. The subunits have been modified in such a way that they will fold into CCMV Virus-Like Particles that have a negative interior. This was done by replacing 6 Arginine and 3 Lysine residues at the N-Terminus with Glutamic acid residues. The promotor and RBS that have been added to the gene allow induced expression of the subunits which increases the yield.
The full protocol for this construct can be found on the Wageningen_UR 2012 Wiki.

Notes

6 Arginine and 3 Lysine residues at the N-Terminus were replaced with Glutamic acid residues.

Source

Cow Chlorotic Mottle Virus (CCMV)

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
B0034	211,222	feature/rbs, ribosome_entry_site
R0010	3,202	feature/promoter, promoter
CCMV with negative N-terminus	229,806	CDS, feature/protein

Other Properties

• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K883161/1

Member of these Collections

• iGEM Parts Registry