BBa_K883201

BBa_K883201 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K883201

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Kees van der Ark

Date created: 2012-09-16 11:00:00

Date modified: 2015-05-08 01:13:40

Hepatitits B core antigen with IPTG induced promotor

Details

• Types: DnaRegion
• Roles: engineered_region; Device
• Sequences: BBa_K883201_sequence (Version 1)

Description

This construct produces Hepatitis B core antigen subunits. These subunits can be used to make HepBcAg Virus-Like Particles. The promotor and RBS that have been added to the gene allow induced expression of the subunits which increases the yield.

The HepBcAg subunits can be easily modified in an external loop.

Notes

The sequence was checked for illegal restriction sites. There were none present. The primers used to amplify the gene contained the prefix and suffix used in the standard 10 assembly.

Source

The parts has been amplified from a plasmid used for the production of HepBcAg in plants: pEAQ-HT-wtHBcAg-EL

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
R0010	1,200	promoter, feature/promoter
B0034	209,220	feature/rbs, ribosome_entry_site
Hepatitis B core Antigen	221,823	feature/protein, CDS

Other Properties

• igem#experience: Works
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K883201/1

Member of these Collections

• iGEM Parts Registry