BBa_K888001

BBa_K888001 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K888001

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Juan Quijano

Date created: 2012-09-23 11:00:00

Date modified: 2015-05-08 01:13:40

AIDA-I translocator domain

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K888001_sequence (Version 1)

Description

AIDA-I is synthetized as a 132 kDa pre-protein featuring a signal peptide which is cleaved during transport trough the inner membrane, a 78 kDa adhesin (passenger) domain, and a 45 kDa translocator. This autotransporter has a large capability in translocating relatively large passengers from 12-65 kDa by showing a N-terminal type of fusion (Li et al. 2007; van Bloois et al. 2011). Coupled with a passenger domain and a signal peptide (K888002), it is possible to express functional proteins in the outer membrane of E. coli

van Bloois et al. (2011). Decorating microbes: surface display of proteins on Escherichia coli. Trends Biotechnol. 29(2): 79-86

Li et al. (2007). Presentation of Functional Organophosphorus Hydrolase Fusions on the Surface of Escherichia coli by the AIDA-I Autotransporter Pathway Biotechnol Bioeng. 99(2): 485-90

Notes

Since the fusion of a passenger domain whit AIDA-I translocator domain, it is required a signal peptide for the passenger expression in the outer membrane

Source

E. coli

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
cds	1,1482	CDS, feature/cds

Other Properties

• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K888001/1

Member of these Collections

• iGEM Parts Registry