BBa_K891234

BBa_K891234 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K891234

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Ryan Muller

Date created: 2012-10-01 11:00:00

Date modified: 2015-05-08 01:13:41

D168A Double Cysteine Mutant of Variola Virus Topoisomerase

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K891234_sequence (Version 1)

Description

This mutant version of topoisomerase recognizes the YCCTT motif in dsDNA. It cleaves after the last T in this motif, making a single stranded nick, and covalently binds to the 3' phosphate on that thymine.

Notes

This part needs to be placed behind an inducible promoter, since it is toxic to the cell and its own plasmid.

Source

This part comes from the smallpox genome. The cysteines have been mutated out and the 168th amino acid has been changed from aspartic acid to alanine. A his tag has been added to the end of this sequence.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
Start codon	1,3	feature/start, start_codon
Stop Codon	942,945	stop_codon, feature/stop
D168A	502,504	feature/mutation, sequence_alteration

Other Properties

• igem#experience: Works
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K891234/1

Member of these Collections

• iGEM Parts Registry