| Types | DnaRegion
|
| Roles | promoter
Regulatory
|
| Sequences | BBa_K895000_sequence (Version 1)
|
Description
The constitutive promoter region (97 bp) from the Escherichia coli K-12 tatABCD (twin-arginine translocase) operon. This is a constitutive promoter that gives good levels of expression in E. coli, but is not inducible or repressible. The promoter region is cloned as an EcoRI / PstI fragment into pSB1C3 following Biobrick Standard [10]. This is a corrected part that replaces the BBa_K562000 biobrick.
Notes
The part has an engineered BamHI (5'-GGATCC-3') restriction site at the extreme 3' end. For best results, open reading frames should be cloned into this BamHI site with their ATG initiation codons immediately following it. This will place the ATG at exactly the correct distance from the native tatA RBS for optimal translational efficiency.
Source
Amplified by PCR from BBa_K562000.
