Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Frank Sargent
Date created: 2012-09-21 11:00:00
Date modified: 2015-05-08 01:13:41
Salty_TtrRS_two_component_system
| Types | DnaRegion |
| Roles | Signalling engineered_region |
| Sequences | BBa_K895003_sequence (Version 1) |
Description
This is a signalling system comprising two open reading frames of the ttrR and ttrS genes together with the promoter/operator of the ttrB gene from Salmonella LT2. Together, TtrR and TtrS form a canonical two-component system where TtrS is the membrane-bound sensor kinase and TtrR is the DNA-binding response regulator. The system senses tetrathionate outside the cell and activates transcription of the ttrB gene, which is part of a tetrathionate reducatase. This part has been designed so that expression of alternative reporter genes can be regulated by tetrathionate.Notes
A PstI site in the natural ttrS sequence has been silently removed.Source
PCR product using Salmonella LT2 genomic DNA as template.| Sequence Annotation | Location | Component / Role(s) |
| ttrR ttrS fnr binding-site RBS transcription start -10 Consensus -35 Consensus promotor for tetrathionate regulon BamHI site | 1,621 596,2275 2397,2410 2542,2548 2475,2475 2462,2468 2438,2443 2351,2555 2550,2555 | CDS feature/cds feature/cds CDS feature/operator operator ribosome_entry_site feature/rbs start_codon feature/start operator feature/operator operator feature/operator feature/promoter promoter sequence_feature feature/dna |