BBa_K895007

BBa_K895007 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K895007

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Frank Sargent

Date created: 2012-09-22 11:00:00

Date modified: 2015-05-08 01:13:41

Tetrathionate-sensing two-component system linked to GFP production.

Details

• Types: DnaRegion
• Roles: Reporter; engineered_region
• Sequences: BBa_K895007_sequence (Version 1)

Description

This is a biosensor comprising the engineered Salmonella TtrRS twon-component system from BBa_K895003 linked to GFP from BBa_K895006, which was originally supplied in BBa_J54103. The concept is that, primarily under anaerobic conditions, the two-component system will sense tetrathionate and induce GFP production.

Notes

The GFP ATG is positioned immediately downstream of an engineered BamHI site. This places the start codon at exactly the correct distance from the ttrB RBS.

Source

This a combination of BBa_K895003 and BBa_K895006 cloned together by PCR.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
ttrR	1,621	CDS, feature/cds
BBa_K895007	1,3275	feature/BioBrick, engineered_region
ttrS	596,2275	feature/cds, CDS
ttrB promoter region	2351,2555	feature/promoter, promoter
RBS	2542,2548	feature/rbs, ribosome_entry_site
BamHI site	2550,2555	feature/dna, sequence_feature
GFP	2556,3275	CDS, feature/cds

Other Properties

• igem#experience: Works
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K895007/1

Member of these Collections

• iGEM Parts Registry