Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Andreas Kj??r
Date created: 2012-09-25 11:00:00
Date modified: 2015-05-08 01:13:42
FFT (BBa_K899001) with double RBS (BBa_K899009)
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K899011_sequence (Version 1) |
Description
This is a composite part containing our fructan:fructan fructosyltransferase (FFT) gene with a "double" RBS containing both the shine-dalgarno and kozak sequence. This is the main part of our project, and also what we submitted to iGEM.The part was characterized in a pJET vector (http://www.bioinfo.pte.hu/f2/pict_f2/pJETmap.pdf) and we found a weird looking substance that resembled sugar structure in the growth medium. When we expressed FFT in E. coli top 10 strains they grew as fast as regular top 10 bacteria, probably because the sucrose:sucrose fructosyltransferase (SST) enzyme wasnt present (this enzyme produces the base that FFT polymerizes).
When staining for inulin we observed a slight increase in inulin even though the SST enzyme wasn't present. This is probably because there was something in the substrate that enabled FFT to polymerize the sugar.
Notes
noneSource
The FFT gene derives from the jerusalem artichoke (Helianthus Tuberosus)| Access | Instance | Definition |
| public public | BBa_K899009 BBa_K899001 | BBa_K899009 BBa_K899001 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K899009 BBa_K899001 | 1,13 20,1867 | BBa_K899009 BBa_K899001 |