Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jeff Addison
Date created: 2012-09-30 11:00:00
Date modified: 2015-05-08 01:13:43
CarAd Coding Sequence
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K902034_sequence (Version 1) |
Description
The CarAd gene in Pseudomonas putida codes for the ferredoxin reductase component of carbazole-1,9-dioxygenase. Together with CarAa and CarAc it forms the carbazole-1,9-dioxygenase (CARDO) enzyme which converts carbazole into 2'-aminobiphenyl-2,3-diol by cleaving one C-N bond in the original carbazole molecule.Notes
Site directed mutagenesis was required to remove a NotI site.Source
Pseudomonas putida plasmid pCAR1.2| Sequence Annotation | Location | Component / Role(s) |
| ATG Start Codon TAA Stop Codon NotI mutation (G --> A) | 1,3 988,990 657,657 | start_codon feature/start feature/stop stop_codon feature/mutation sequence_alteration |